Biotinylated Human MRGPRX2 Nanodisc

Name: Biotinylated Human MRGPRX2 Nanodisc
Brand: 普维拓生物
SKU: MRGPRX2-HB002

货号MRGPRX2-HB002

HumanHEK293分子名 · MRGX2理论分子量 · 50.1 kDa

产品简介

Recombinant Biotinylated Human MRGPRX2 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Val330 (UniProt accession: Q96LB1).

产品详情

分子别名: MRGX2
蛋白长度: Met1-Val330
表达系统: HEK293
理论分子量: The protein has a predicted MW of 50.1 kDa.
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
储存条件: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
阴性对照: CON-HM1NB

数据展示

ELISA Data — Immobilized Biotinylated Human MRGPRX2 Nanodisc, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-MRGPRX2 Antibody, mFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test).

BLI Data — Loaded Biotinylated Human MRGPRX2 Nanodisc, His Tag on Streptavidin-Biosensor can bind Anti-MRGPRX2 Antibody, mFc Tag with an affinity constant of 0.25 nM as determined in BLI assay.

背景介绍

As receptor for multiple drugs, MRGPRX2 is crucial to drug-induced hypersensitivity. However, not only drugs, but also endogenous mediators like neuropeptides and host defense peptides activate MRGPRX2, suggesting its broad impact in cutaneous pathophysiology.

文献引用

1.Wang Z, Babina M. MRGPRX2 signals its importance in cutaneous mast cell biology: Does MRGPRX2 connect mast cells and atopic dermatitis? Exp Dermatol. 2020 Nov;29(11):1104-1111. doi: 10.1111/exd.14182. Epub 2020 Sep 17. PMID: 32866307.

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Biotinylated Human MRGPRX2 Nanodisc

产品简介

产品详情

数据展示

ELISA

ELISA Data

BLI

BLI Data

背景介绍

文献引用

Biotinylated Human MRGPRX2 Nanodisc

产品简介

产品详情

数据展示

ELISA

ELISA Data

BLI

BLI Data

背景介绍

文献引用