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免疫检查点蛋白

免疫检查点是维持免疫耐受、限制过度免疫反应并调节 T 细胞效应功能的关键受体—配体系统。抗原呈递细胞或肿瘤细胞表面的配体与 T 细胞受体结合后，可传递共刺激或共抑制信号，共同决定 T 细胞的活化、增殖、效应和耗竭状态。

PD-1/PD-L1、CTLA-4/CD80/CD86、TIGIT/CD155、TIM-3/Galectin-9 与 LAG-3/MHC II 等通路广泛用于肿瘤免疫、炎症与自身免疫研究。本系列提供多种物种、标签形式及表达体系的重组蛋白，可用于受体—配体结合验证、抗体筛选、亲和力与动力学分析以及细胞功能研究。

研究方向肿瘤免疫 · 免疫耐受 · T 细胞调控核心应用结合检测 · 抗体发现 · 功能验证

免疫检查点相互作用图谱

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免疫检查点相互作用图中的免疫细胞树突状细胞T细胞肿瘤细胞巨噬细胞

产品特点与应用领域

靶点蛋白产品列表

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共抑制性检查点10 个分子
BTLACD155CD172aCD47CD86HVEMPD1PDL1SIRPATIGIT
共刺激性检查点5 个分子
4-1BB41BBCD134CD28GITR
其他相关分子16 个分子
B7H3B7H4CD112CD150CD172bCD172gCD226CD244CD276CD4CD85dCD85jCD85kNECTIN4PVRIGSIRPB

免疫检查点蛋白产品

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应用案例与参考检测数据

Antibody Binding

PD-1 与 PVRIG 抗体结合活性

ELISA dose-response curve for nivolumab binding to human PD-1
Nivolumab showed concentration-dependent binding to immobilized human PD-1 in ELISA, with a reported EC50 of 1.31 ng/mL.
ELISA dose-response curve for an anti-PVRIG antibody
A human anti-PVRIG antibody generated a concentration-dependent ELISA signal against immobilized PVRIG, confirming target-specific recognition.

Affinity and Kinetics

PVRIG—NECTIN2 受体配体结合与动力学

ELISA dose-response curve for PVRIG binding to NECTIN2
PVRIG displayed concentration-dependent binding to immobilized human NECTIN2 in ELISA, supporting functional receptor-ligand recognition.
SPR sensorgrams for the interaction between PVRIG and NECTIN2
Real-time SPR sensorgrams recorded the association and dissociation phases of the PVRIG-NECTIN2 interaction across a serial concentration range.

Antibody Discovery and Screening

TIGIT—CD155 中和抗体筛选

ELISA inhibition curve for an anti-TIGIT antibody blocking CD155 binding
Serially diluted anti-TIGIT antibody inhibited the TIGIT-CD155 interaction in a competitive ELISA, with a reported IC50 of approximately 0.56 µg/mL.
Flow cytometry inhibition curve for an anti-TIGIT antibody
Increasing concentrations of an anti-TIGIT monoclonal antibody reduced the cell-associated CD155 binding signal in a competitive flow cytometry assay.

Cell Binding and Functional Studies

CD47—SIRPα 细胞结合与 4-1BBL 功能活性

Flow cytometry histogram showing CD47 binding to SIRP alpha on ACHN cells
Flow cytometry detected binding of human CD47 protein to SIRP-alpha expressed on ACHN cells at a test concentration of 10 µg/mL.
Flow cytometry inhibition curve for antibody-mediated blockade of CD47 binding
A neutralizing SIRP-alpha antibody reduced the cell-associated CD47 binding signal as its concentration increased in a competitive flow cytometry assay.
Cell-based dose-response curve for 4-1BB ligand functional activity
Human 4-1BB ligand stimulated CD137-expressing cells and produced a dose-dependent IL-8 response, providing a cell-based functional activity readout.