Biotinylated Human MRGPRX2 Nanodisc

Name: Biotinylated Human MRGPRX2 Nanodisc
Brand: Puweituo Bio
SKU: MRGPRX2-HB002

Catalog numberMRGPRX2-HB002

HumanHEK293Molecule · MRGX2Molecular weight · 50.1 kDa

Product introduction

Recombinant Biotinylated Human MRGPRX2 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Val330 (UniProt accession: Q96LB1).

Product details

Molecular aliases: MRGX2
Protein length: Met1-Val330
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 50.1 kDa.
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Storage: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Negative control: CON-HM1NB

Data display

ELISA Data — Immobilized Biotinylated Human MRGPRX2 Nanodisc, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-MRGPRX2 Antibody, mFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test).

BLI Data — Loaded Biotinylated Human MRGPRX2 Nanodisc, His Tag on Streptavidin-Biosensor can bind Anti-MRGPRX2 Antibody, mFc Tag with an affinity constant of 0.25 nM as determined in BLI assay.

Background

As receptor for multiple drugs, MRGPRX2 is crucial to drug-induced hypersensitivity. However, not only drugs, but also endogenous mediators like neuropeptides and host defense peptides activate MRGPRX2, suggesting its broad impact in cutaneous pathophysiology.

References

1.Wang Z, Babina M. MRGPRX2 signals its importance in cutaneous mast cell biology: Does MRGPRX2 connect mast cells and atopic dermatitis? Exp Dermatol. 2020 Nov;29(11):1104-1111. doi: 10.1111/exd.14182. Epub 2020 Sep 17. PMID: 32866307.

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Biotinylated Human MRGPRX2 Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

BLI

BLI Data

Background

References

Biotinylated Human MRGPRX2 Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

BLI

BLI Data

Background

References