Human A2AR Nanodisc Protein

Name: Human A2AR Nanodisc Protein
Brand: 普维拓生物
SKU: A2AR-HP001

货号A2AR-HP001

HEK293分子名 · A2aR理论分子量 · 45.5 kDa

产品简介

Recombinant Human A2AR Nanodisc protein is expressed in HEK293 cells with a C-terminal His tag and contains Met1-Ser412 (UniProt accession: P29274). The nanodisc format presents the full-length multi-pass membrane protein in a membrane-like environment for antibody-binding and assay-development research.

产品详情

分子别名: A2aR; Adenosine A2a R; ADORA 2; ADORA2; ADORA2A; RDC8
蛋白长度: Met1-Ser412
表达系统: HEK293
理论分子量: 45.5 kDa
内毒素: <1 EU/μg (LAL assay)
缓冲液 / 制剂: 0.22 μm filtered solution in PBS with 200 mM L-arginine, pH 7.4. Not recommended for flow cytometry in mammalian cells.
储存条件: Store at -80°C for up to 6 months. Minimize freeze-thaw cycles.
阴性对照: CON-HM1NB

数据展示

ELISA Data — Immobilized Human A2AR Nanodisc, His Tag at 2 μg/mL (100 μL/well) on the plate. The dose-response curve for Anti-A2AR Antibody, mFc Tag gave an EC50 of 0.15 μg/mL by ELISA (QC test).

SPR Data — Human A2AR Nanodisc, His Tag captured on a CM5 chip via anti-His antibody bound Anti-A2AR Antibody, mFc Tag with an affinity constant of 0.32 nM by SPR (Biacore T200).

BLI Data — Anti-A2AR Antibody loaded on a Protein A biosensor bound Human A2AR Nanodisc, His Tag with an affinity constant of 4.44 nM by BLI (Gator).

背景介绍

Adenosine is a neuromodulator in the adult central nervous system. Membrane-bound receptors for adenosine include the A1, A2A, A2B and A3 receptor subtypes. Expression of A2A adenosine receptor mRNA in cranial ganglia, the carotid body and the intermediate lobe of the pituitary gland suggests additional sites of adenosine action during development and in adults.

文献引用

1.Weaver DR. A2a adenosine receptor gene expression in developing rat brain. Brain Res Mol Brain Res. 1993 Dec;20(4):313-327. doi: 10.1016/0169-328X(93)90058-W. PMID: 8114618.

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Human A2AR Nanodisc Protein

货号A2AR-HP001

HEK293分子名 · A2aR理论分子量 · 45.5 kDa

Human A2AR Nanodisc Protein

产品简介

产品详情

数据展示

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

背景介绍

文献引用

Human A2AR Nanodisc Protein

产品简介

产品详情

数据展示

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

背景介绍

文献引用