Human CD47 Protein

Name: Human CD47 Protein
Brand: 普维拓生物
SKU: CD47-HP001

货号CD47-HP001

HumanHEK293分子名 · CD47 glycoprotein理论分子量 · 14.5 kDa

产品简介

Recombinant Human CD47 is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Gln19-Pro139 (UniProt accession: Q08722-1).

产品详情

分子别名: CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6
蛋白长度: Gln19-Pro139
表达系统: HEK293
理论分子量: The protein has a predicted MW of 14.5 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human CD47 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human CD47 is greater than 90% as determined by SEC-HPLC.

ELISA Data — Immobilized Human CD47, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 82.1ng/ml determined by ELISA (QC Test).

SPR Data — Human CD47, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human SIRP alpha, hFc Tag with an affinity constant of 18.9nM as determined in SPR assay (Biacore T200).

背景介绍

CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.

文献引用

1.Weiskopf K, Ring A M, Ho C C M, et al. Engineered SIRP? Variants as Immunotherapeutic Adjuvants to Anticancer Antibodies[J]. Science, 2013, 341(6141):88-91. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.

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Human CD47 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

背景介绍

文献引用

Human CD47 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

背景介绍

文献引用