Human A2AR Nanodisc Protein

Name: Human A2AR Nanodisc Protein
Brand: Puweituo Bio
SKU: A2AR-HP001

Catalog numberA2AR-HP001

HEK293Molecule · A2aRMolecular weight · 45.5 kDa

Product introduction

Recombinant Human A2AR Nanodisc protein is expressed in HEK293 cells with a C-terminal His tag and contains Met1-Ser412 (UniProt accession: P29274). The nanodisc format presents the full-length multi-pass membrane protein in a membrane-like environment for antibody-binding and assay-development research.

Product details

Molecular aliases: A2aR; Adenosine A2a R; ADORA 2; ADORA2; ADORA2A; RDC8
Protein length: Met1-Ser412
Expression system: HEK293
Molecular weight: 45.5 kDa
Endotoxin: <1 EU/μg (LAL assay)
Formulation: 0.22 μm filtered solution in PBS with 200 mM L-arginine, pH 7.4. Not recommended for flow cytometry in mammalian cells.
Storage: Store at -80°C for up to 6 months. Minimize freeze-thaw cycles.
Negative control: CON-HM1NB

Data display

ELISA Data — Immobilized Human A2AR Nanodisc, His Tag at 2 μg/mL (100 μL/well) on the plate. The dose-response curve for Anti-A2AR Antibody, mFc Tag gave an EC50 of 0.15 μg/mL by ELISA (QC test).

SPR Data — Human A2AR Nanodisc, His Tag captured on a CM5 chip via anti-His antibody bound Anti-A2AR Antibody, mFc Tag with an affinity constant of 0.32 nM by SPR (Biacore T200).

BLI Data — Anti-A2AR Antibody loaded on a Protein A biosensor bound Human A2AR Nanodisc, His Tag with an affinity constant of 4.44 nM by BLI (Gator).

Background

Adenosine is a neuromodulator in the adult central nervous system. Membrane-bound receptors for adenosine include the A1, A2A, A2B and A3 receptor subtypes. Expression of A2A adenosine receptor mRNA in cranial ganglia, the carotid body and the intermediate lobe of the pituitary gland suggests additional sites of adenosine action during development and in adults.

References

1.Weaver DR. A2a adenosine receptor gene expression in developing rat brain. Brain Res Mol Brain Res. 1993 Dec;20(4):313-327. doi: 10.1016/0169-328X(93)90058-W. PMID: 8114618.

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Human A2AR Nanodisc Protein

Catalog numberA2AR-HP001

HEK293Molecule · A2aRMolecular weight · 45.5 kDa

Human A2AR Nanodisc Protein

Product introduction

Product details

Data display

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

Background

References

Human A2AR Nanodisc Protein

Product introduction

Product details

Data display

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

Background

References