Human Wnt Surrogate-Fc Fusion Protein

Name: Human Wnt Surrogate-Fc Fusion Protein
Brand: 普维拓生物
SKU: WNTSURROGATE-HF001

货号WNTSURROGATE-HF001

HumanHEK293分子名 · Wnt-3a理论分子量 · 58.50 kDa

产品简介

Recombinant Human Wnt Surrogate-Fc Fusion Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus

产品详情

分子别名: Wnt-3a; Wnt 3a; WNT3A protein; Wnt3a
表达系统: HEK293
理论分子量: The protein has a predicted MW of 58.50 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human Wnt Surrogate-Fc Fusion on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Cell Based Assay Data — Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 2-12 ng/mL (QC Test).

背景介绍

Wnt-3a is regarded as an activator of the canonical Wnt signaling pathway. This activator is expressed in the dorsal midline region and is responsible for spinal cord development. In addition, Wnt-3a plays a regulatory role in autophagy, apoptosis, and regeneration of neurons, neurogenic inflammation and axon regeneration.Wnt-3a promotes the beta-catenin/Tcf pathway which is tumor inducing and can cause cancer when expressed in particular cell populations. It is also one of the most commonly used cytokines for organoid construction.

文献引用

1.Gao K, Zhang T, Wang F, Lv C. Therapeutic Potential of Wnt-3a in Neurological Recovery after Spinal Cord Injury. Eur Neurol. 2019;81(3-4):197-204. doi: 10.1159/000502004. Epub 2019 Jul
2.PMID: 31336380.
3.Pashirzad M, Fiuji H, Khazei M, Moradi-Binabaj M, Ryzhikov M, Shabani M, Avan A, Hassanian SM. Role of Wnt3a in the pathogenesis of cancer, current status and prospective. Mol Biol Rep. 2019 Oct;46(5):5609-5616. doi: 10.1007/s11033-019-04895-4. Epub 2019 Jun
4.PMID: 31236761.

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Human Wnt Surrogate-Fc Fusion Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

Bioactivity

Cell Based Assay Data

背景介绍

文献引用

Human Wnt Surrogate-Fc Fusion Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

Bioactivity

Cell Based Assay Data

背景介绍

文献引用