Biotinylated Human VEGF-C/Flt4-L Protein

Name: Biotinylated Human VEGF-C/Flt4-L Protein
Brand: 普维拓生物
SKU: VEGFC-HB001

货号VEGFC-HB001

HumanHEK293分子名 · VEGFC理论分子量 · 17.1 kDa

产品简介

Recombinant Biotinylated Human VEGF-C/Flt4-L Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Thr103-Arg227 (UniProt accession: Q6FH59).

产品详情

分子别名: VEGFC; VEGFc; Flt4-L; VRP; VEGF-C;Flt4L
蛋白长度: Thr103-Arg227
表达系统: HEK293
理论分子量: The protein has a predicted MW of 17.1 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22μm filtered solution in 50mM MES, 150mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in 50mM MES (pH 6.0). Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Biotinylated Human VEGF-C on Bis-Tris PAGE under reduced (R) condition. The purity is greater than 95%.

ELISA Data — Immobilized Biotinylated Human VEGF-C, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA.

背景介绍

the lymphangiogenic factors vascular endothelial growth factor C (VEGFC) and VEGFD are cleaved by thrombin and plasmin, serine proteases generated during hemostasis and wound healing. Genetic studies reveal that platelet enhancement of lymphatic growth after wounding is dependent on the release of VEGFC, but not VEGFD, a finding consistent with high expression of VEGFC in both platelets and avian thrombocytes.

文献引用

1.Lim L, Bui H, Farrelly O, Yang J, Li L, Enis D, Ma W, Chen M, Oliver G, Welsh JD, Kahn ML. Hemostasis stimulates lymphangiogenesis through release and activation of VEGFC. Blood. 2019 Nov 14;134(20):1764-1775. doi: 10.1182/blood.2019001736. PMID: 31562136; PMCID: PMC6856989.

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Biotinylated Human VEGF-C/Flt4-L Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

背景介绍

文献引用

Biotinylated Human VEGF-C/Flt4-L Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

背景介绍

文献引用