Human Mature TGF beta 1 Protein

Name: Human Mature TGF beta 1 Protein
Brand: 普维拓生物
SKU: TGFB1-HP001

货号TGFB1-HP001

HumanHEK293分子名 · CEDLAP理论分子量 · 13.2 kDa

产品简介

Recombinant Human Mature TGF beta 1 Protein is expressed in HEK293 cells without tag. It contains amino acid residues Ala279-Ser390 (UniProt accession: P01137).

产品详情

分子别名: CEDLAP; DPD1; TGFB1; TGF-β1; TGF-beta 1
蛋白长度: Ala279-Ser390
表达系统: HEK293
理论分子量: The protein has a predicted MW of 13.2 kDa (monomer). Due to glycosylation, the protein migrates to 14-15 kDa (monomer) under reduced (R) condition and 26-30 kDa (dimer) under Non reduced (N) condition based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22μm filtered solution in 50mM Glycine 150mM NaCl (pH 2.5). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in 4mM HCL. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human Mature TGF beta 1 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.

ELISA Data — Immobilized Human Mature TGF beta 1, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).

SPR Data — Human TGFBR1, mFc Tag captured on CM5 Chip via Protein A can bind Human Mature TGF beta 1, No Tag with an affinity constant of 0.35 μM as determined in SPR assay (Biacore T200).

Cell Proliferation Bioactivity — Measured by its ability to inhibit cell proliferation of Mv-1-lu mink lung epithelial cells. The ED50 for this effect is typically 0.1-0.2 ng/mL.

背景介绍

TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition.Transforming growth factor beta-1 is multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration.

文献引用

1.Robertson I B, Rifkin D B. Regulation of the Bioavailability of TGF-β and TGF-β-Related Proteins[J]. Cold Spring Harbor Perspectives in Biology, 2016, 8(6):a021907.

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Human Mature TGF beta 1 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

SPR

SPR Data

Bioactivity

Cell Proliferation Bioactivity

背景介绍

文献引用

Human Mature TGF beta 1 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

SPR

SPR Data

Bioactivity

Cell Proliferation Bioactivity

背景介绍

文献引用