Biotinylated Human Notch 1 Protein

Name: Biotinylated Human Notch 1 Protein
Brand: 普维拓生物
SKU: NOTCH1-HB001

货号NOTCH1-HB001

HumanHEK293分子名 · Notch-1理论分子量 · 56.5 kDa

产品简介

Recombinant Biotinylated Human Notch 1 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ala19-Gln526 (UniProt accession: P46531).

产品详情

分子别名: Notch-1; TAN1; NOTCH1; 9930111A19Rik; lin-12; Mis6; N1;lin12
蛋白长度: Ala19-Gln526
表达系统: HEK293
理论分子量: The protein has a predicted MW of 56.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Biotinylated Human Notch 1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human Notch 1 is greater than 90% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human Notch 1, His-Avi tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human DLL4, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA (QC Test).

SPR Data — Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Notch 1, His Tag with an affinity constant of 0.31 μM as determined in SPR assay (Biacore T200).

背景介绍

Human Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes. Notch-1 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

文献引用

1.Hales E C, Taub J W, Matherly L H. New insights into Notch1 regulation of the PI3K–AKT–mTOR1 signaling axis: Targeted therapy of γ-secretase inhibitor resistant T-cell acute lymphoblastic leukemia[J]. Cellular Signalling, 2014, 26(1):149-161.

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Biotinylated Human Notch 1 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

SPR

SPR Data

背景介绍

文献引用

Biotinylated Human Notch 1 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

SPR

SPR Data

背景介绍

文献引用