Human Noggin Protein

Name: Human Noggin Protein
Brand: 普维拓生物
SKU: NOGGIN-HP001

货号NOGGIN-HP001

HumanCHO分子名 · Noggin; NOG; SYM1; SYNS1理论分子量 · 23.05 kDa

产品简介

Recombinant Human Noggin Protein is expressed in CHO cells without tag. It contains amino acid residues Gln28-Cys232 (UniProt accession: Q13253).

产品详情

分子别名: Noggin; NOG; SYM1; SYNS1
蛋白长度: Gln28-Cys232
表达系统: CHO
理论分子量: The protein has a predicted MW of 23.05 kDa. Due to glycosylation, the protein migrates to 30-36 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 0.05 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human Noggin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human Noggin is greater than 95% as determined by SEC-HPLC.

Cell Based Assay Data — Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 4-80 ng/mL in the presence of 50 ng/mL of recombinant Human BMP‑4.

背景介绍

Noggin is an antagonist of bone morphogenetic proteins (BMP), being indispensable for certain developmental events.Noggin expression positively correlated with EGFR expression in both GC cell line models and The Cancer Genome Atlas human GC cohort. Targeting EGFR and its downstream pathways diminished cell proliferation which was promoted by Noggin. Noggin promotes the proliferation of GC cells by upregulating EGFR and enhancing a vicious circle formed by β‑catenin, EGFR, ERK and Akt.

文献引用

1.Sun Z, Gao X, Zabkiewicz C, Ruge F, Xie M, Cai S, Sun PH, Griffiths P, Pugh MR, Ji J, Jiang WG, Ye L. Noggin is associated with a poor prognosis of gastric cancer by promoting the proliferation of gastric cancer cells via the upregulation of EGFR. Int J Oncol. 2020 Sep;57(3):813-824. doi: 10.3892/ijo.2020.5081. Epub 2020 Jun
2.PMID: 32705152.

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Human Noggin Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Bioactivity

Cell Based Assay Data

背景介绍

文献引用

Human Noggin Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Bioactivity

Cell Based Assay Data

背景介绍

文献引用