Human GDNF Protein

Name: Human GDNF Protein
Brand: 普维拓生物
SKU: GDNF-HP001

货号GDNF-HP001

HumanE. coli分子名 · Glial cell line-derived neurotrophic factor理论分子量 · 15.06 kDa

产品简介

Recombinant Human GDNF Protein is expressed in E. coli without tagIt contains Ser78-Ile211 (UniProt accession: P39905-1).

产品详情

分子别名: Glial cell line-derived neurotrophic factor; hGDNF; Astrocyte-derived trophic factor (ATF)
蛋白长度: Ser78-Ile211
表达系统: E. coli
理论分子量: The protein has a predicted MW of 15.06 kDa same as Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 0.1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human GDNF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human GDNF is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human GDNF, No Tag at 1 μg/ml (100μl/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).

Cell Proliferation Bioactivity — Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is 2‑12 ng/mL in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera. (QC Test)

背景介绍

Glial cell line-derived neurotrophic factor (GDNF) is a 134 amino acid protein belonging in the GDNF family ligands (GFLs). GDNF was originally isolated from rat glial cell lines and identified as a neurotrophic factor with the ability to promote dopamine uptake within midbrain dopaminergic neurons.

文献引用

1.PMID: 32897420; PMCID: PMC7529617.

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Human GDNF Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Bioactivity

Cell Proliferation Bioactivity

背景介绍

文献引用

Human GDNF Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Bioactivity

Cell Proliferation Bioactivity

背景介绍

文献引用