Human FGF basic Protein

Name: Human FGF basic Protein
Brand: 普维拓生物
SKU: FGFBASIC-HP001

货号FGFBASIC-HP001

HumanE. coli分子名 · bFGF理论分子量 · 16.5 kDa

产品简介

Recombinant Human FGF basic Protein is expressed in E. coli without tag It contains amino acid residues Pro143-Ser288 (UniProt accession: P09038-4).

产品详情

分子别名: bFGF; FGF basic; FGF2; FGF-2; FGF2AS; GFG1; HBGF-2; NUDT6; Prostatropin; BFGF; FGFB; FGFβ; HBGF2
蛋白长度: Pro143-Ser288
表达系统: E. coli
理论分子量: The protein has a predicted MW of 16.5 kDa same as Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 0.02 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22 μm filtered solution in PBS, 8% trehalose (pH 7.4).
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human FGF basic on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human FGF basic is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human FGF basic at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 11.1ng/ml determined by ELISA. (QC Test)

Cell Proliferation Bioactivity — Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is 0.1 - 0.6 ng/ml (QC Test).

背景介绍

FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway.

文献引用

1.Zhiwu J, Xiaofeng J, Suimin C, et al. Anti-GPC3-CAR T Cells Suppress the Growth of Tumor Cells in Patient-Derived Xenografts of Hepatocellular Carcinoma[J]. Frontiers in Immunology, 2017, 7.
2.Ho M, Kim H. Glypican-3: A new target for cancer immunotherapy[J]. European Journal of Cancer, 2011, 47(3):0-338.

加载中…

Human FGF basic Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

Bioactivity

Cell Proliferation Bioactivity

背景介绍

文献引用

Human FGF basic Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

Bioactivity

Cell Proliferation Bioactivity

背景介绍

文献引用