Biotinylated Human FGF10 Protein (Primary Amine Labeling)

Name: Biotinylated Human FGF10 Protein (Primary Amine Labeling)
Brand: 普维拓生物
SKU: FGF10-HB001

货号FGF10-HB001

HumanE. coli分子名 · FGF-10理论分子量 · 19.3 kDa

产品简介

Recombinant Biotinylated Human FGF10 Protein (Primary Amine Labeling) is expressed in E. coli without tag. It contains amino acid residues Gln38-Ser208 (UniProt accession: O15520).

产品详情

分子别名: FGF-10; KGF2; FGF10; Fibroblast growth factor-10; Fibroblast growth factor 10
蛋白长度: Gln38-Ser208
表达系统: E. coli
理论分子量: The protein has a predicted MW of 19.3 kDa same as Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Biotinylated Human FGF10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human FGF10 is greater than 95% as determined by SEC-HPLC.

SPR Data — Biotinylated Human FGF10, No tag immobilized on CM5 Chip can bind Human FGFR2 alpha IIIb, His tag with an affinity constant of 147.70 nM as determined in SPR assay (Biacore T200) (QC Test).

Blocking Data — Serial dilutions of Anti-FGFR2 alpha (IIIb) Antibody were added into Human FGFR2 alpha (IIIb), His Tag: Biotinylated Human FGF10, No Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.7ng/ml.

背景介绍

Fibroblast growth factor 10 (FGF10) regulates multiple stages of structural lung morphogenesis, cellular differentiation, and the response to injury. As a driver of lung airway branching morphogenesis, FGF10 signaling defects during development lead to neonatal lung disease. Lung diseases impact patients across the lifespan, from infants in the first minutes of life through the aged population. Congenital abnormalities of lung structure can cause lung disease at birth or make adults more susceptible to chronic disease.

文献引用

1.Prince LS. FGF10 and Human Lung Disease Across the Life Spectrum. Front Genet. 2018 Oct 31;9:517. doi: 10.3389/fgene.2018.00517. PMID: 30429870; PMCID: PMC6220039.

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Biotinylated Human FGF10 Protein (Primary Amine Labeling)

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SPR

SPR Data

Blocking

Blocking Data

背景介绍

文献引用

Biotinylated Human FGF10 Protein (Primary Amine Labeling)

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SPR

SPR Data

Blocking

Blocking Data

背景介绍

文献引用