Biotinylated Human Claudin 3 Nanodisc

Name: Biotinylated Human Claudin 3 Nanodisc
Brand: 普维拓生物
SKU: CLDN3-HB002

货号CLDN3-HB002

HumanHEK293分子名 · Claudin-3理论分子量 · 36.4 kDa

产品简介

Recombinant Biotinylated Human Claudin 3 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Val220 (UniProt accession: O15551).

产品详情

分子别名: Claudin-3; CPE-R 2; CPE-receptor 2; hRVP1; CLDN3; C7orf1; CPETR2; RVP1
蛋白长度: Met1-Val220
表达系统: HEK293
理论分子量: The protein has a predicted MW of 36.4 kDa.
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
储存条件: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
阴性对照: CON-HM1NB

数据展示

ELISA Data — Immobilized Biotinylated Human Claudin 3, His Tag at 2 μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Claudin 3 Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. (QC Test)

SPR Data — Biotinylated Human Claudin 3 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-Claudin 3 Antibody, hFc Tag with an affinity constant of 24.48 nM as determined in SPR assay (Biacore T200).

背景介绍

Claudin-3 is a memember of the Claudin family, which is present in many epithelia including skin, lung, kidney and intestine. It beautifully localizes on the cell-to-cell junctions of MCF-7 cells, clearly forming a continuous belt-like structure surrounding the cells. It has often been associated with the progression and metastasis of various tumors.

文献引用

1.Milatz S, Krug SM, Rosenthal R, Günzel D, Müller D, Schulzke JD, Amasheh S, Fromm M. Claudin-3 acts as a sealing component of the tight junction for ions of either charge and uncharged solutes. Biochim Biophys Acta. 2010 Nov;1798(11):2048-57. doi: 10.1016/j.bbamem.2010.07.014. Epub 2010 Jul 22. PMID: 20655293.

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Biotinylated Human Claudin 3 Nanodisc

产品简介

产品详情

数据展示

ELISA

ELISA Data

SPR

SPR Data

背景介绍

文献引用

Biotinylated Human Claudin 3 Nanodisc

产品简介

产品详情

数据展示

ELISA

ELISA Data

SPR

SPR Data

背景介绍

文献引用