Biotinylated Human Claudin 3 Nanodisc

Name: Biotinylated Human Claudin 3 Nanodisc
Brand: Puweituo Bio
SKU: CLDN3-HB002

Catalog numberCLDN3-HB002

HumanHEK293Molecule · Claudin-3Molecular weight · 36.4 kDa

Product introduction

Recombinant Biotinylated Human Claudin 3 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Val220 (UniProt accession: O15551).

Product details

Molecular aliases: Claudin-3; CPE-R 2; CPE-receptor 2; hRVP1; CLDN3; C7orf1; CPETR2; RVP1
Protein length: Met1-Val220
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 36.4 kDa.
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Storage: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Negative control: CON-HM1NB

Data display

ELISA Data — Immobilized Biotinylated Human Claudin 3, His Tag at 2 μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Claudin 3 Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. (QC Test)

SPR Data — Biotinylated Human Claudin 3 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-Claudin 3 Antibody, hFc Tag with an affinity constant of 24.48 nM as determined in SPR assay (Biacore T200).

Background

Claudin-3 is a memember of the Claudin family, which is present in many epithelia including skin, lung, kidney and intestine. It beautifully localizes on the cell-to-cell junctions of MCF-7 cells, clearly forming a continuous belt-like structure surrounding the cells. It has often been associated with the progression and metastasis of various tumors.

References

1.Milatz S, Krug SM, Rosenthal R, Günzel D, Müller D, Schulzke JD, Amasheh S, Fromm M. Claudin-3 acts as a sealing component of the tight junction for ions of either charge and uncharged solutes. Biochim Biophys Acta. 2010 Nov;1798(11):2048-57. doi: 10.1016/j.bbamem.2010.07.014. Epub 2010 Jul 22. PMID: 20655293.

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Biotinylated Human Claudin 3 Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

SPR

SPR Data

Background

References

Biotinylated Human Claudin 3 Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

SPR

SPR Data

Background

References