Biotinylated Human CD20/MS4A1 Protein

Name: Biotinylated Human CD20/MS4A1 Protein
Brand: 普维拓生物
SKU: CD20-HB005

货号CD20-HB005

HumanE. coli分子名 · MS4A1理论分子量 · 16.8 kDa

产品简介

Recombinant Biotinylated Human CD20/MS4A1 Protein is expressed in E. coli with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ile141-Ser188 (UniProt accession: P11836-1).

产品详情

分子别名: MS4A1; CD20; MS4A-1; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 11; Leukocyte surface antigen Leu16
蛋白长度: Ile141-Ser188
表达系统: E. coli
理论分子量: The protein has a predicted MW of 16.8 kDa same as Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Supplied as 0.22μm filtered solution in 20mM PB, 500mM NaCl, 0.4M Urea, 10% glycerol, 1mM GSH, 0.1mM GSSG (pH 7.0).
储存条件: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

数据展示

Bis-Tris PAGE Data — Biotinylated Human CD20 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data — Immobilized Biotinylated Human CD20, His-Avi tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD20 Antibody, hFc Tag with the EC50 of 0.13μg/ml determined by ELISA (QC Test).

BLI Data — Loaded Biotinylated Human CD20, His-Avi tag on Steptavidin-Biosensor can bind Anti-CD20 Antibody, hFc Tag with an affinity constant of 0.15 μM as determined in BLI assay.

背景介绍

B-lymphocyte antigen CD20 or CD20 is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R, CD117) and progressively increasing in concentration until maturity. CD20 is the target of the monoclonal antibodies rituximab, ocrelizumab, obinutuzumab, ofatumumab, ibritumomab tiuxetan, tositumomab, and ublituximab, which are all active agents in the treatment of all B cell lymphomas, leukemias, and B cell-mediated autoimmune diseases.

文献引用

1.Ma D, Mcdevitt M R, Barendswaard E, et al. Radioimmunotherapy for model B cell malignancies using 90Y-labeled anti-CD19 and anti-CD20 monoclonal antibodies[J]. Leukemia, 2002, 16(1):60-66.
2.Jager U, Fridrik M, Zeitlinger M, et al. Rituximab serum concentrations during immuno-chemotherapy of follicular lymphoma correlate with patient gender, bone marrow infiltration and clinical response[J]. Haematologica, 2012, 97(9):1431-1438.

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Biotinylated Human CD20/MS4A1 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

BLI

BLI Data

背景介绍

文献引用

Biotinylated Human CD20/MS4A1 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

BLI

BLI Data

背景介绍

文献引用