Biotinylated Human CD20/MS4A1 Protein

Name: Biotinylated Human CD20/MS4A1 Protein
Brand: Puweituo Bio
SKU: CD20-HB005

Catalog numberCD20-HB005

HumanE. coliMolecule · MS4A1Molecular weight · 16.8 kDa

Product introduction

Recombinant Biotinylated Human CD20/MS4A1 Protein is expressed in E. coli with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ile141-Ser188 (UniProt accession: P11836-1).

Product details

Molecular aliases: MS4A1; CD20; MS4A-1; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 11; Leukocyte surface antigen Leu16
Protein length: Ile141-Ser188
Expression system: E. coli
Molecular weight: The protein has a predicted MW of 16.8 kDa same as Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22μm filtered solution in 20mM PB, 500mM NaCl, 0.4M Urea, 10% glycerol, 1mM GSH, 0.1mM GSSG (pH 7.0).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Biotinylated Human CD20 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data — Immobilized Biotinylated Human CD20, His-Avi tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD20 Antibody, hFc Tag with the EC50 of 0.13μg/ml determined by ELISA (QC Test).

BLI Data — Loaded Biotinylated Human CD20, His-Avi tag on Steptavidin-Biosensor can bind Anti-CD20 Antibody, hFc Tag with an affinity constant of 0.15 μM as determined in BLI assay.

Background

B-lymphocyte antigen CD20 or CD20 is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R, CD117) and progressively increasing in concentration until maturity. CD20 is the target of the monoclonal antibodies rituximab, ocrelizumab, obinutuzumab, ofatumumab, ibritumomab tiuxetan, tositumomab, and ublituximab, which are all active agents in the treatment of all B cell lymphomas, leukemias, and B cell-mediated autoimmune diseases.

References

1.Ma D, Mcdevitt M R, Barendswaard E, et al. Radioimmunotherapy for model B cell malignancies using 90Y-labeled anti-CD19 and anti-CD20 monoclonal antibodies[J]. Leukemia, 2002, 16(1):60-66.
2.Jager U, Fridrik M, Zeitlinger M, et al. Rituximab serum concentrations during immuno-chemotherapy of follicular lymphoma correlate with patient gender, bone marrow infiltration and clinical response[J]. Haematologica, 2012, 97(9):1431-1438.

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Biotinylated Human CD20/MS4A1 Protein

Catalog numberCD20-HB005

HumanE. coliMolecule · MS4A1Molecular weight · 16.8 kDa

Product introduction

Recombinant Biotinylated Human CD20/MS4A1 Protein is expressed in E. coli with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ile141-Ser188 (UniProt accession: P11836-1).

Biotinylated Human CD20/MS4A1 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

BLI

BLI Data

Background

References

Biotinylated Human CD20/MS4A1 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

BLI

BLI Data

Background

References