Human VEGF165 Protein

Name: Human VEGF165 Protein
Brand: 普维拓生物
SKU: VEGF165-HP001

货号VEGF165-HP001

HumanHEK293分子名 · VEGF理论分子量 · 19.2 kDa

产品简介

Recombinant Human VEGF165 Protein is expressed in HEK293 cells without tag. It contains amino acid residues Ala27-Arg191 (UniProt accession: P15692-4).

产品详情

分子别名: VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609;VEGF-165
蛋白长度: Ala27-Arg191
表达系统: HEK293
理论分子量: The protein has a predicted MW of 19.2 kDa. Due to glycosylation, the protein migrates to 20-30 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 0.05 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human VEGF165 on Bis-Tris PAGE under reduced condition and Non reduced (N) condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human VEGF165 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human VEGF165 at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 46.4ng/ml determined by ELISA. (QC Test)

Blocking Data — Serial dilutions of Anti-VEGF165 Antibody were added into Human VEGF165, No Tag: Biotinylated Human VEGF R2, His Avi tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.30μg/ml.

Cell Based Assay Data — Measured by a reporter gene assay using HEK293T-KDR-NFAT Cell line. The ED50 for this effect is < 10 ng/mL (QC Test).

背景介绍

Human papillomavirus (HPV) infection is an established risk factor for cervical carcinogenesis. VEGF165 was significantly higher, whereas VEGFC and VEGFD were significantly lower in malignant cervical carcinoma tissues as compared to normal cervix tissues. Expression levels of VEGF121 and VEGFC were significantly associated with type of tumor growth while VEGF165 was significantly associated with lymph node metastasis.

文献引用

1.Patel KA, Patel BM, Thobias AR, Gokani RA, Chhikara AB, Desai AD, Patel PS. Overexpression of VEGF165 is associated with poor prognosis of cervical cancer. J Obstet Gynaecol Res. 2020 Nov;46(11):2397-2406. doi: 10.1111/jog.14483. Epub 2020 Sep
2.PMID: 32985053.

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Human VEGF165 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

ELISA

ELISA Data

ELISA

ELISA Data

Blocking

Blocking Data

Bioactivity

Cell Based Assay Data

背景介绍

文献引用

Human VEGF165 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

ELISA

ELISA Data

ELISA

ELISA Data

Blocking

Blocking Data

Bioactivity

Cell Based Assay Data

背景介绍

文献引用