Rat TIGIT Protein

Name: Rat TIGIT Protein
Brand: 普维拓生物
SKU: TIGIT-RP001

货号TIGIT-RP001

RatHEK293分子名 · TIGIT理论分子量 · 39.47 kDa

产品简介

Recombinant Rat TIGIT Protein is expressed in HEK293 cells with an mFc (IgG2a) tag at the C-terminus. It contains amino acid residues Ala17-Gln136 (accession: D3ZTQ2).

产品详情

分子别名: TIGIT; VSIG9; VSTM3; WUCAM
蛋白长度: Ala17-Gln136
表达系统: HEK293
理论分子量: The protein has a predicted MW of 39.47 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Rat TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Rat TIGIT is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Mouse CD155, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Rat TIGIT, mFc Tag with the EC50 of 23.1ng/ml determined by ELISA. (QC Test)

BLI Data — Loaded Mouse CD155, His Tag on Anti-His-Biosensor can bind Rat TIGIT, mFc Tag with an affinity constant of 6.93 nM as determined in BLI assay.

背景介绍

TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.

文献引用

1.Yu X, Harden K, C Gonzalez L, et al. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells[J]. NATURE IMMUNOLOGY, 2008, 10(1):48-57. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.

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Rat TIGIT Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

BLI

BLI Data

背景介绍

文献引用

Rat TIGIT Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

BLI

BLI Data

背景介绍

文献引用