Biotinylated Human/Cynomolgus Activin RIIB/ACVR2B Protein

Name: Biotinylated Human/Cynomolgus Activin RIIB/ACVR2B Protein
Brand: 普维拓生物
SKU: RIIB-HF004

货号RIIB-HF004

HumanHEK293分子名 · Activin receptor type-2B理论分子量 · 41.41 kDa

产品简介

Recombinant Biotinylated Human/Cynomolgus Activin RIIB/ACVR2B Protein is expressed in HEK293 cells with an hFc (IgG1) tag and Avi tag at the C-terminus. It contains amino acid residues Ser19-Thr137 (Human) / Ser40-Thr158 (Cynomolgus) (accession: Q13705-1(Human) / XP_045242398.1(Cynomolgus)).

产品详情

分子别名: Activin receptor type-2B; ACVR2B; Activin RIIB; ActRIIB
蛋白长度: Ser19-Thr137
表达系统: HEK293
理论分子量: The protein has a predicted MW of 41.41 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Biotinylated Human/Cynomolgus Activin RIIB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human/Cynomolgus Activin RIIB is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human Activin A, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 20.9ng/ml determined by ELISA (QC Test).

背景介绍

ActRIIB (activin receptor type-2B) is an activin receptor subtype constitutively expressed in the whole body, playing a role in cellular proliferation, differentiation, and metabolism. For its various physiological activities, ActRIIB interacts with activin and multiple other ligands including myostatin (MSTN), growth differentiation factor 11 (GDF11), and bone morphogenetic protein 9 (BMP9).

文献引用

1.Sakamoto K, Kanematsu-Yamaki Y, Kamada Y, Oka M, Ohnishi T, Miwa M, Asami T, Inooka H. Identification of ligand-selective peptidic ActRIIB-antagonists using phage display technology. Biochem Biophys Rep. 2017 Jun 10;11:33-39. doi: 10.1016/j.bbrep.2017.06.001. PMID: 28955765; PMCID: PMC5614685.

加载中…

Biotinylated Human/Cynomolgus Activin RIIB/ACVR2B Protein

货号RIIB-HF004

HumanHEK293分子名 · Activin receptor type-2B理论分子量 · 41.41 kDa

Biotinylated Human/Cynomolgus Activin RIIB/ACVR2B Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

背景介绍

文献引用

Biotinylated Human/Cynomolgus Activin RIIB/ACVR2B Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

背景介绍

文献引用