PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein

Name: PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein
Brand: 普维拓生物
SKU: PRAME-HP006

货号PRAME-HP006

HumanHEK293分子名 · PRAME

产品简介

Recombinant PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer is assembled by biotinylated monomer and PE-labeled streptavidin. It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and SLLQHLIGL peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &SLLQHLIGL).

产品详情

分子别名: PRAME; OIP-4; OIP4; MAPE;
蛋白长度: Gly25-Thr305
表达系统: HEK293
缓冲液 / 制剂: Supplied as 0.22 μm filtered solution in PBS, 0.2% BSA (pH 7.4).
储存条件: Valid for 6 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

数据展示

ELISA Data — Immobilized PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 46.9ng/ml determined by ELISA.

Flow Cytometry Data — 1E6 of PRAME specific TCR-HEK293T cell line were stained with 100ul of 10ug/ml PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer (Cat. No.MHC-HM443TP) and non-transfected HEK293T cells and PE-Labeled protein were used as negative control. The binding activity was evaluated by PE signal. (QC Test)

背景介绍

PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.

文献引用

1.Lezcano C, Jungbluth AA, Busam KJ. PRAME Immunohistochemistry as an Ancillary Test for the Assessment of Melanocytic Lesions. Surg Pathol Clin. 2021 Jun;14(2):165-175. doi: 10.1016/j.path.2021.01.001. Epub 2021 Apr 28. PMID: 34023098; PMCID: PMC8152939.

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PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein

货号PRAME-HP006

HumanHEK293分子名 · PRAME

PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein

产品简介

产品详情

数据展示

ELISA

ELISA Data

Flow Cytometry

Flow Cytometry Data

背景介绍

文献引用

PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein

产品简介

产品详情

数据展示

ELISA

ELISA Data

Flow Cytometry

Flow Cytometry Data

背景介绍

文献引用