Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein

Name: Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein
Brand: 普维拓生物
SKU: PRAME-HB004

货号PRAME-HB004

HumanE. coli分子名 · PRAME理论分子量 · 35.6 kDa

产品简介

Recombinant Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLQHLIGL peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &SLLQHLIGL).

产品详情

分子别名: PRAME; OIP-4; OIP4; MAPE;
蛋白长度: Gly25-Thr305
表达系统: E. coli
理论分子量: The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
储存条件: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

数据展示

Bis-Tris PAGE Data — Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer, His Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for HLA-A*02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. (QC Test)

背景介绍

PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.

文献引用

1.Lezcano C, Jungbluth AA, Busam KJ. PRAME Immunohistochemistry as an Ancillary Test for the Assessment of Melanocytic Lesions. Surg Pathol Clin. 2021 Jun;14(2):165-175. doi: 10.1016/j.path.2021.01.001. Epub 2021 Apr 28. PMID: 34023098; PMCID: PMC8152939.

加载中…

Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein

货号PRAME-HB004

HumanE. coli分子名 · PRAME理论分子量 · 35.6 kDa

Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用

Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用