Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein

Name: Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein
Brand: 普维拓生物
SKU: PRAME-HP005

货号PRAME-HP005

HumanHEK293分子名 · PRAME理论分子量 · 258 kDa

产品简介

Recombinant Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and SLLQHLIGL peptide (accession: A0A140T913 (HLA-A*02:01) & P61769 (B2M) &SLLQHLIGL).

产品详情

分子别名: PRAME; OIP-4; OIP4; MAPE;
蛋白长度: Gly25-Thr305
表达系统: HEK293
理论分子量: The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR with the EC50 of 9.9 ng/ml determined by ELISA. (QC Test)

BLI Data — Loaded HLA-A*02:01&B2M&PRAME TCR, hFc Tag on ProA-Biosensor can bind Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer with an affinity constant of 48.80 pM as determined in BLI assay.

背景介绍

PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.

文献引用

1.Lezcano C, Jungbluth AA, Busam KJ. PRAME Immunohistochemistry as an Ancillary Test for the Assessment of Melanocytic Lesions. Surg Pathol Clin. 2021 Jun;14(2):165-175. doi: 10.1016/j.path.2021.01.001. Epub 2021 Apr 28. PMID: 34023098; PMCID: PMC8152939.

加载中…

Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein

货号PRAME-HP005

HumanHEK293分子名 · PRAME理论分子量 · 258 kDa

Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

BLI

BLI Data

背景介绍

文献引用

Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

BLI

BLI Data

背景介绍

文献引用