Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein

Name: Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein
Brand: Puweituo Bio
SKU: PRAME-HB004

Catalog numberPRAME-HB004

HumanE. coliMolecule · PRAMEMolecular weight · 35.6 kDa

Product introduction

Recombinant Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLQHLIGL peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &SLLQHLIGL).

Product details

Molecular aliases: PRAME; OIP-4; OIP4; MAPE;
Protein length: Gly25-Thr305
Expression system: E. coli
Molecular weight: The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer, His Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for HLA-A*02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. (QC Test)

Background

PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.

References

1.Lezcano C, Jungbluth AA, Busam KJ. PRAME Immunohistochemistry as an Ancillary Test for the Assessment of Melanocytic Lesions. Surg Pathol Clin. 2021 Jun;14(2):165-175. doi: 10.1016/j.path.2021.01.001. Epub 2021 Apr 28. PMID: 34023098; PMCID: PMC8152939.

加载中…

Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein

Catalog numberPRAME-HB004

HumanE. coliMolecule · PRAMEMolecular weight · 35.6 kDa

Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References