Biotinylated Canine PD-L1/B7-H1 Protein

Name: Biotinylated Canine PD-L1/B7-H1 Protein
Brand: 普维拓生物
SKU: PDL1-DB001

货号PDL1-DB001

HEK293分子名 · CD274理论分子量 · 52.91 kDa

产品简介

Recombinant Biotinylated Canine PD-L1/B7-H1 Protein is expressed in HEK293 cells with an mFc tag and Avi tag at the C-terminus. It contains amino acid residues Phe19-Arg236 (accession: E2RKZ5).

产品详情

分子别名: CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1
蛋白长度: Phe19-Arg236
表达系统: HEK293
理论分子量: The protein has a predicted MW of 52.91 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Biotinylated Canine PD-L1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Canine PD-L1 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Canine PD-L1, mFc Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Canine PD-1, hFc Tag (Cat. PD1-DM201) with the EC50 of 53.3ng/ml determined by ELISA. (QC Test)

背景介绍

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.

文献引用

1.Blank C, Mackensen A. Contribution of the PD-L1/PD-1 pathway to T-cell exhaustion: an update on implications for chronic infections and tumor evasion[J]. Cancer Immunology Immunotherapy, 2007, 56(5):739-745. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.

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Biotinylated Canine PD-L1/B7-H1 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用

Biotinylated Canine PD-L1/B7-H1 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用