Biotinylated Nanodisc Control

Name: Biotinylated Nanodisc Control
Brand: 普维拓生物
SKU: NANODISCCTRL-HB001

货号NANODISCCTRL-HB001

RatHEK293分子名 · Nanodisc; Control理论分子量 · 24.0 kDa

产品简介

Recombinant Biotinylated Nanodisc Control is expressed in HEK293 cells with a His tag at the C-terminus. Our Control Nanodisc features a small, integrated alpha-helix peptide at the center of the nanodisc. The phospholipid disc is stabilized by an SMA (a type of copolymer) belt as the scaffold. The control nanodiscs are used to exclude experimental artifacts caused by the scaffold or phospholipid components, such as fluorescent background or nonspecific interactions

产品详情

分子别名: Nanodisc; Control
表达系统: HEK293
理论分子量: The protein has a predicted MW of 24.0 kDa.
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
储存条件: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

背景介绍

SMA based nanodiscs are synthetic structures formed using styrene maleic anhydride (SMA), which allows for the stabilization and solubilization of membrane proteins without the use of detergents. The advantage is directly extraction proteins from their native cell membrane environment.

文献引用

1.Detergent-Free Membrane Protein Purification Using SMA Polymer. Methods Mol Biol. 2022;2507:389-404. doi: 10.1007/978-1-0716-2368-8_21.
2.Factors influencing the detergent-free membrane protein isolation using synthetic nanodisc-forming polymers. Biochim Biophys Acta Biomembr. 2024 Jan;1866(1):184240. doi: 10.1016/j.bbamem.2023.184240.

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