Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc

Name: Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc
Brand: 普维拓生物
SKU: KRASG12D-HB001

货号KRASG12D-HB001

HumanHEK293分子名 · KRAS G12D TCR&CD3理论分子量 · 196.0 kDa

产品简介

Recombinant Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc is expressed from HEK293. Both the biotin and the His tag are located on the C-terminus of the KRAS G12D TCR. It contains KRAS G12D specific TCR (JD1a41b1), CD3ε&CD3γ, CD3ε&CD3δ, and CD3ζ dimers

产品详情

分子别名: KRAS G12D TCR&CD3
表达系统: HEK293
理论分子量: The protein has a predicted MW of 196.0 kDa.
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry.
储存条件: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
阴性对照: CON-HM1NB

数据展示

ELISA Data — Immobilized Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 17.5ng/ml determined by ELISA. (QC Test)

SPR Data — Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind OKT3, mFc Tag with an affinity constant of 0.21 nM as determined in SPR assay (Biacore T200).

BLI Data — Loaded Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag on Streptavidin-Biosensor can bind OKT3, mFc Tag with an affinity constant of 0.90 nM as determined in BLI assay.

背景介绍

The JD1a41b1 TCR is an engineered, high-affinity T-cell receptor that strictly recognizes the peptide fragment of KRAS G12D (VVVGADGVGK) presented on the HLA-A*11:01 only. Cluster of Differentiation 3 (CD3) is a multimeric protein complex and T cell co-receptor that plays an essential role in adaptive immune responses. These CD3 dimers bind non-covalently to the TCR to form the TCR&CD3 complex on the cell surface.

文献引用

1.(1) Poole, Andrew, et al. "Therapeutic high affinity T cell receptor targeting a KRASG12D cancer neoantigen." Nature communications 13.1 (2022): 5333. DOI: 10.1038/s41467-022-32811-1. PMID: 36088370, PMCID: PMC9464187

加载中…

Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc

货号KRASG12D-HB001

HumanHEK293分子名 · KRAS G12D TCR&CD3理论分子量 · 196.0 kDa

Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc

产品简介

产品详情

数据展示

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

SPR

SPR Data

BLI

BLI Data

BLI

BLI Data

背景介绍

文献引用

Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc

产品简介

产品详情

数据展示

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

SPR

SPR Data

BLI

BLI Data

BLI

BLI Data

背景介绍

文献引用