Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc

Name: Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc
Brand: Puweituo Bio
SKU: KRASG12D-HB001

Catalog numberKRASG12D-HB001

HumanHEK293Molecule · KRAS G12D TCR&CD3Molecular weight · 196.0 kDa

Product introduction

Recombinant Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc is expressed from HEK293. Both the biotin and the His tag are located on the C-terminus of the KRAS G12D TCR. It contains KRAS G12D specific TCR (JD1a41b1), CD3ε&CD3γ, CD3ε&CD3δ, and CD3ζ dimers

Product details

Molecular aliases: KRAS G12D TCR&CD3
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 196.0 kDa.
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry.
Storage: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Negative control: CON-HM1NB

Data display

ELISA Data — Immobilized Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 17.5ng/ml determined by ELISA. (QC Test)

SPR Data — Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind OKT3, mFc Tag with an affinity constant of 0.21 nM as determined in SPR assay (Biacore T200).

BLI Data — Loaded Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag on Streptavidin-Biosensor can bind OKT3, mFc Tag with an affinity constant of 0.90 nM as determined in BLI assay.

Background

The JD1a41b1 TCR is an engineered, high-affinity T-cell receptor that strictly recognizes the peptide fragment of KRAS G12D (VVVGADGVGK) presented on the HLA-A*11:01 only. Cluster of Differentiation 3 (CD3) is a multimeric protein complex and T cell co-receptor that plays an essential role in adaptive immune responses. These CD3 dimers bind non-covalently to the TCR to form the TCR&CD3 complex on the cell surface.

References

1.(1) Poole, Andrew, et al. "Therapeutic high affinity T cell receptor targeting a KRASG12D cancer neoantigen." Nature communications 13.1 (2022): 5333. DOI: 10.1038/s41467-022-32811-1. PMID: 36088370, PMCID: PMC9464187

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Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc

Catalog numberKRASG12D-HB001

HumanHEK293Molecule · KRAS G12D TCR&CD3Molecular weight · 196.0 kDa

Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

SPR

SPR Data

BLI

BLI Data

BLI

BLI Data

Background

References

Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

SPR

SPR Data

BLI

BLI Data

BLI

BLI Data

Background

References