Human IL-4 Protein

Name: Human IL-4 Protein
Brand: 普维拓生物
SKU: IL4-HP001

货号IL4-HP001

HumanHEK293分子名 · IL4理论分子量 · 14.96 kDa

产品简介

Recombinant Human IL-4 Protein is expressed in HEK293 cells without tag. It contains amino acid residues His25-Ser153 (UniProt accession: P05112-1).

产品详情

分子别名: IL4; IL-4; interleukin 4; MGC79402; pitrakinra; B cell growth factor 1; BCDF; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; BSF-1
蛋白长度: His25-Ser153
表达系统: HEK293
理论分子量: The protein has a predicted MW of 14.96 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human IL-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human IL-4 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human IL-4, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag (Cat. ILA-HM24R) with the EC50 of 4.4ng/ml determined by ELISA. (QC Test)

BLI Data — Loaded Human IL-4 R alpha, hFc Tag (Cat. ILA-HM24R) on ProA-Biosensor can bind Human IL-4, No Tag with an affinity constant of 0.54 nM as determined in BLI assay.

Cell Proliferation Bioactivity — Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.01-0.35 ng/mL (QC Test)

背景介绍

Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation.In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor.

文献引用

1.Ghoreschi K, Thomas P, Breit S, et al. Interleukin-4 therapy of psoriasis induces Th2 responses and improves human autoimmune disease[J]. Nature Medicine, 2002, 9(1):40-46.

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Human IL-4 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

BLI

BLI Data

Bioactivity

Cell Proliferation Bioactivity

背景介绍

文献引用

Human IL-4 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

BLI

BLI Data

Bioactivity

Cell Proliferation Bioactivity

背景介绍

文献引用