Human IL-33 Protein

Name: Human IL-33 Protein
Brand: 普维拓生物
SKU: IL33-HP001

货号IL33-HP001

HumanE. coli分子名 · C9orf26 Z理论分子量 · 18 kDa

产品简介

Recombinant Human IL-33 Protein is expressed in E. coli without tag. It contains amino acid residues Ser112-Thr270 (accession: NP_254274.1).

产品详情

分子别名: C9orf26 Z; DVS27; IL1F11; NF-HEV; NFEHEV; IL33; DV27; C9ORF26; RP11-575C20.2
蛋白长度: Ser112-Thr270
表达系统: E. coli
理论分子量: The protein has a predicted MW of 18 kDa same as Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human IL-33 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human IL-33 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human IL-33, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-1RL1, His Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test).

SPR Data — Human IL-1RL1, hFc Tag (Cat. ST2-HM2L1) captured on CM5 Chip via Protein A can bind Human IL-33, No Tag with an affinity constant of 0.11 nM as determined in SPR assay (Biacore T200).

Cell Based Assay Data — Measured by a reporter gene assay using IL33 Effector Reporter Cell line. The ED50 for this effect is < 0.5 ng/mL.

背景介绍

Interleukin-33 (IL-33) is a cytokine belonging to the IL-1 family, playing a role in inflammatory, infectious and autoimmune diseases and expressed in the cellular nucleus in several tissues. High levels of IL-33 are expressed in epithelial barrier tissues and endothelial barriers. ST2 is a receptor for IL-33, expressed selectively on a subset of Th2 cells, mediating some of their functions.

文献引用

1.Liew FY, Girard JP, Turnquist HR. Interleukin-33 in health and disease. Nat Rev Immunol. 2016;16(11):676-689. doi:10.1038/nri.2016.95

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Human IL-33 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

Bioactivity

Cell Based Assay Data

背景介绍

文献引用

Human IL-33 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

ELISA

ELISA Data

ELISA

ELISA Data

SPR

SPR Data

Bioactivity

Cell Based Assay Data

背景介绍

文献引用