Human IL-21 Protein

Name: Human IL-21 Protein
Brand: 普维拓生物
SKU: IL21-HP001

货号IL21-HP001

HumanE. coli分子名 · Interleukin-21理论分子量 · 15.4 kDa

产品简介

Recombinant Human IL-21 Protein is expressed in E. coli without tag. It contains amino acid residues Gln32-Ser162 (UniProt accession: Q9HBE4-1).

产品详情

分子别名: Interleukin-21; IL-21; Za11; CVID11
蛋白长度: Gln32-Ser162
表达系统: E. coli
理论分子量: The protein has a predicted MW of 15.4 kDa same as Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22μm filtered solution in 20mM NaAc, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in 20mM NaAc (pH 5.5). Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human IL-21 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human IL-21 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human IL-21, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-21R, hFc Tag with the EC50 of 42.3ng/ml determined by ELISA. (QC Test)

Cell Based Assay Data — Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is <8 ng/mL (QC Test).

背景介绍

Interleukin-21 (IL-21), produced predominantly by CD4 T cells and natural killer T (NKT) cells, is a newly discovered member of the common γ-chain family of cytokines. It has been implicated in many immunological processes and has been linked to autoimmune diseases, allergies and other inflammatory diseases.

文献引用

1.Sim GC, Radvanyi L. The IL-2 cytokine family in cancer immunotherapy. Cytokine Growth Factor Rev. 2014 Aug;25(4):377-90. doi: 10.1016/j.cytogfr.2014.07.018. Epub 2014 Aug
2.PMID: 25200249.

加载中…

Human IL-21 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Bioactivity

Cell Based Assay Data

背景介绍

文献引用

Human IL-21 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Bioactivity

Cell Based Assay Data

背景介绍

文献引用