Human IL-18 Protein

Name: Human IL-18 Protein
Brand: 普维拓生物
SKU: IL18-HP001

货号IL18-HP001

HumanE. coli分子名 · IL-18理论分子量 · 18.2 kDa

产品简介

Recombinant Human IL-18 Protein is expressed in E. coli without tag. It contains amino acid residues Tyr37-Asp193 (UniProt accession: Q14116-1).

产品详情

分子别名: IL-18; IL18; IGIF; IL-1F4; IL-1 gamma; IL1F4; IL-1g; Interleukin 1; IL-1γ; IL-1 γ
蛋白长度: Tyr37-Asp193
表达系统: E. coli
理论分子量: The protein has a predicted MW of 18.2 kDa same as Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 0.1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human IL-18 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human IL-18 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human IL-18 at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag (Cat. IL8-HM2BP) with the EC50 of 62.0ng/ml determined by ELISA (QC Test).

SPR Data — Human IL-18 R1, His Tag (Cat. IL8-HM4R1) captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 2.38 nM as determined in SPR assay (Biacore T200).

BLI Data — Loaded Human IL-18BP, His Tag (Cat. IL8-HM1BP) on Anti-His-Biosensor can bind Human IL-18, No Tag with an affinity constant of 1.80 nM as determined in BLI assay.

Cell Based Assay Data — Measured by a reporter gene assay. The ED50 for this effect is 10-50 pg/mL. (QC Test)

背景介绍

Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.

文献引用

1.Yamada T, Aoyama-Ishikawa M, Yamashita H, Fujiwara M, Usami M, Ueda T, Terashima M, Kohama K, Nakao A, Kotani J. IL-18 production and IL18 promoter polymorphisms correlate with mortality in ICU patients. In Vivo. 2014 May-Jun;28(3):391-6. PMID: 24815843.

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Human IL-18 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

Bioactivity

Cell Based Assay Data

背景介绍

文献引用

Human IL-18 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

Bioactivity

Cell Based Assay Data

背景介绍

文献引用