GMP Human IL-15

Name: GMP Human IL-15
Brand: 普维拓生物
SKU: IL15-HP002

货号IL15-HP002

HumanE. coli分子名 · Interleukin-15理论分子量 · 12.8 kDa

产品简介

Recombinant GMP Human IL-15 is expressed in E. coli without tag. It contains amino acid residues Asn49-Ser162 (UniProt accession: P40933-1).

产品详情

分子别名: Interleukin-15; IL-15; IL15; il 15; MGC9721
蛋白长度: Asn49-Ser162
表达系统: E. coli
理论分子量: The protein has a predicted MW of 12.8 kDa same as Bis-Tris PAGE result.
纯度: ≥ 95% as determined by SEC-HPLC.
内毒素: ≤ 0.01 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: The product is shipped with ice bag. Store it immediately at -20°C±5°C upon arrival. -20°C±5°C for 36 months in lyophilized state. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

数据展示

Bis-Tris PAGE Data — GMP Human IL-15 on Bis-Tris PAGE under reduced condition.

37°C Accelerated Stability — GMP Human IL-15 (reconstituted protein) was stored at 37°C, the protein is stable for 7 days determined by cell based assay.

4°C Accelerated Stability — GMP Human IL-15 (reconstituted protein) was stored at 4°C, the protein is stable for 90 days determined by cell based assay.

Freeze-Thaw Stability — GMP Human IL-15 (reconstituted protein) was repeatedly frozen at -80°C and thawed at 37°C, the protein is stable after freezing and thawing 3 cycles determined by cell based assay.

Cell Proliferation Bioactivity — Measured in a cell proliferation assay using CTLL-2 cells. The ED50 for this effect is 0.1 - 0.3 ng/mL. The specific activity of GMP Human IL-15 is > 1 × 10^7 IU/mg, which is calibrated against the human IL-15 WHO reference standard (NIBSC code: 95/554) (QC Test).

背景介绍

IL-15 belongs to the common gamma chain cytokine family and has pleiotropic immunological functions, and the protein is mainly produced by monocytes, dendritic cells, and epithelial cells. IL-15 plays a crucial role in the development, homeostasis, and function of T, NK and NK-T cells, and is additionally required for various functions of the B cells, dendritic cells (DC), macrophages, and mast cells. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.

文献引用

1.Lee H, Park SH, Shin EC. IL-15 in T-Cell Responses and Immunopathogenesis. Immune Netw. 2024 Feb 16;24(1):e11.
2.Patidar M, Yadav N, Dalai SK. Interleukin 15: A key cytokine for immunotherapy. Cytokine Growth Factor Rev. 2016 Oct;31:49-59.

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GMP Human IL-15

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

Stability

37°C Accelerated Stability

Stability

4°C Accelerated Stability

Stability

Freeze-Thaw Stability

Bioactivity

Cell Proliferation Bioactivity

背景介绍

文献引用

GMP Human IL-15

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

Stability

37°C Accelerated Stability

Stability

4°C Accelerated Stability

Stability

Freeze-Thaw Stability

Bioactivity

Cell Proliferation Bioactivity

背景介绍

文献引用