GMP Human IL-15

Name: GMP Human IL-15
Brand: Puweituo Bio
SKU: IL15-HP002

Catalog numberIL15-HP002

HumanE. coliMolecule · Interleukin-15Molecular weight · 12.8 kDa

Product introduction

Recombinant GMP Human IL-15 is expressed in E. coli without tag. It contains amino acid residues Asn49-Ser162 (UniProt accession: P40933-1).

Product details

Molecular aliases: Interleukin-15; IL-15; IL15; il 15; MGC9721
Protein length: Asn49-Ser162
Expression system: E. coli
Molecular weight: The protein has a predicted MW of 12.8 kDa same as Bis-Tris PAGE result.
Purity: ≥ 95% as determined by SEC-HPLC.
Endotoxin: ≤ 0.01 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: The product is shipped with ice bag. Store it immediately at -20°C±5°C upon arrival. -20°C±5°C for 36 months in lyophilized state. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — GMP Human IL-15 on Bis-Tris PAGE under reduced condition.

37°C Accelerated Stability — GMP Human IL-15 (reconstituted protein) was stored at 37°C, the protein is stable for 7 days determined by cell based assay.

4°C Accelerated Stability — GMP Human IL-15 (reconstituted protein) was stored at 4°C, the protein is stable for 90 days determined by cell based assay.

Freeze-Thaw Stability — GMP Human IL-15 (reconstituted protein) was repeatedly frozen at -80°C and thawed at 37°C, the protein is stable after freezing and thawing 3 cycles determined by cell based assay.

Cell Proliferation Bioactivity — Measured in a cell proliferation assay using CTLL-2 cells. The ED50 for this effect is 0.1 - 0.3 ng/mL. The specific activity of GMP Human IL-15 is > 1 × 10^7 IU/mg, which is calibrated against the human IL-15 WHO reference standard (NIBSC code: 95/554) (QC Test).

Background

IL-15 belongs to the common gamma chain cytokine family and has pleiotropic immunological functions, and the protein is mainly produced by monocytes, dendritic cells, and epithelial cells. IL-15 plays a crucial role in the development, homeostasis, and function of T, NK and NK-T cells, and is additionally required for various functions of the B cells, dendritic cells (DC), macrophages, and mast cells. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.

References

1.Lee H, Park SH, Shin EC. IL-15 in T-Cell Responses and Immunopathogenesis. Immune Netw. 2024 Feb 16;24(1):e11.
2.Patidar M, Yadav N, Dalai SK. Interleukin 15: A key cytokine for immunotherapy. Cytokine Growth Factor Rev. 2016 Oct;31:49-59.

加载中…

GMP Human IL-15

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

Stability

37°C Accelerated Stability

Stability

4°C Accelerated Stability

Stability

Freeze-Thaw Stability

Bioactivity

Cell Proliferation Bioactivity

Background

References

GMP Human IL-15

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

Stability

37°C Accelerated Stability

Stability

4°C Accelerated Stability

Stability

Freeze-Thaw Stability

Bioactivity

Cell Proliferation Bioactivity

Background

References