Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer Protein

Name: Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer Protein
Brand: 普维拓生物
SKU: HLAA0201-HB005

货号HLAA0201-HB005

HumanE. coli分子名 · PLK-1; STPK13; PLK理论分子量 · 35.6 kDa

产品简介

Recombinant Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and AAPGAPAAA peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &AAPGAPAAA).

产品详情

分子别名: PLK-1; STPK13; PLK
蛋白长度: Gly25-Thr305
表达系统: E. coli
理论分子量: The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
储存条件: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

数据展示

Bis-Tris PAGE Data — Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer, His Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 14.0ng/ml determined by ELISA.

背景介绍

A key regulator of cell division is polo-like kinase 1 (PLK1), a member of the serine/threonine kinase family that plays an important role in regulating the mitotic and meiotic cell cycle. The phosphorylation of specific substrates mediated by PLK1 controls nuclear envelope breakdown (NEBD), centrosome maturation, proper spindle assembly, chromosome segregation, and cytokinesis.

文献引用

1.Kalous J, Aleshkina D. Multiple Roles of PLK1 in Mitosis and Meiosis. Cells. 2023 Jan 2;12(1):187. doi: 10.3390/cells12010187. PMID: 36611980; PMCID: PMC9818836.

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Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer Protein

货号HLAA0201-HB005

HumanE. coli分子名 · PLK-1; STPK13; PLK理论分子量 · 35.6 kDa

Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用

Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用