Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer Protein

Name: Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer Protein
Brand: Puweituo Bio
SKU: HLAA0201-HB005

Catalog numberHLAA0201-HB005

HumanE. coliMolecule · PLK-1; STPK13; PLKMolecular weight · 35.6 kDa

Product introduction

Recombinant Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and AAPGAPAAA peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &AAPGAPAAA).

Product details

Molecular aliases: PLK-1; STPK13; PLK
Protein length: Gly25-Thr305
Expression system: E. coli
Molecular weight: The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer, His Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 14.0ng/ml determined by ELISA.

Background

A key regulator of cell division is polo-like kinase 1 (PLK1), a member of the serine/threonine kinase family that plays an important role in regulating the mitotic and meiotic cell cycle. The phosphorylation of specific substrates mediated by PLK1 controls nuclear envelope breakdown (NEBD), centrosome maturation, proper spindle assembly, chromosome segregation, and cytokinesis.

References

1.Kalous J, Aleshkina D. Multiple Roles of PLK1 in Mitosis and Meiosis. Cells. 2023 Jan 2;12(1):187. doi: 10.3390/cells12010187. PMID: 36611980; PMCID: PMC9818836.

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Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer Protein

Catalog numberHLAA0201-HB005

HumanE. coliMolecule · PLK-1; STPK13; PLKMolecular weight · 35.6 kDa

Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Human HLA-A*02:01&B2M&PLK1 (AAPGAPAAA) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References