Human HLA-A*02:01&B2M&HIV Gag (SLYNTVATL) Monomer Protein

Name: Human HLA-A*02:01&B2M&HIV Gag (SLYNTVATL) Monomer Protein
Brand: 普维拓生物
SKU: HIVGAG-HP001

货号HIVGAG-HP001

HumanHEK293分子名 · HIV; HIV Gag; SLYNTVATL理论分子量 · 50.39 kDa

产品简介

Recombinant Human HLA-A*02:01&B2M&HIV Gag (SLYNTVATL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and SLYNTVATL peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &SLYNTVATL).

产品详情

分子别名: HIV; HIV Gag; SLYNTVATL
蛋白长度: Gly25-Thr305
表达系统: HEK293
理论分子量: The protein has a predicted MW of 50.39 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human HLA-A*02:01&B2M&HIV Gag (SLYNTVATL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human HLA-A*02:01&B2M&HIV Gag (SLYNTVATL) Monomer is greater than 95% as determined by SEC-HPLC.

背景介绍

Gag is the HIV structural precursor protein which is cleaved by viral protease to produce mature infectious viruses. Gag is a polyprotein composed of MA (matrix), CA (capsid), SP1, NC (nucleocapsid), SP2 and p6 domains. The immunodominant HLA-A2-restricted Gag epitope, SLYNTVATL (SL9), is considered to be a poor immunogen because reactivity to it is rare in acute infection despite its paradoxical dominance in patients with chronic infection.

文献引用

1.(1) Mendonça L, Sun D, Ning J, Liu J, Kotecha A, Olek M, Frosio T, Fu X, Himes BA, Kleinpeter AB, Freed EO, Zhou J, Aiken C, Zhang P. CryoET structures of immature HIV Gag reveal six-helix bundle. Commun Biol. 2021 Apr 16;4(1):481. doi: 10.1038/s42003-021-01999-1. PMID: 33863979; PMCID: PMC8052356. (2) Kan-Mitchell J, Bajcz M, Schaubert KL, Price DA, Brenchley JM, Asher TE, Douek DC, Ng HL, Yang OO, Rinaldo CR Jr, Benito JM, Bisikirska B, Hegde R, Marincola FM, Boggiano C, Wilson D, Abrams J, Blondelle SE, Wilson DB. Degeneracy and repertoire of the human HIV-1 Gag p17(77-85) CTL response. J Immunol. 2006 Jun 1;176(11):6690-701. doi: 10.4049/jimmunol.176.11.6690. PMID: 16709828.

加载中…

Human HLA-A*02:01&B2M&HIV Gag (SLYNTVATL) Monomer Protein

货号HIVGAG-HP001

HumanHEK293分子名 · HIV; HIV Gag; SLYNTVATL理论分子量 · 50.39 kDa

Human HLA-A*02:01&B2M&HIV Gag (SLYNTVATL) Monomer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

背景介绍

文献引用

Human HLA-A*02:01&B2M&HIV Gag (SLYNTVATL) Monomer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

背景介绍

文献引用