Biotinylated Human GIPR Nanodisc

Name: Biotinylated Human GIPR Nanodisc
Brand: 普维拓生物
SKU: GIPR-HB003

货号GIPR-HB003

HumanHEK293分子名 · GIP-R; GIPR; PGQTL2理论分子量 · 63.8 kDa

产品简介

Recombinant Biotinylated Human GIPR Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Cys466 (UniProt accession: P48546-1).

产品详情

分子别名: GIP-R; GIPR; PGQTL2
蛋白长度: Met1-Cys466
表达系统: HEK293
理论分子量: The protein has a predicted MW of 63.8 kDa.
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
储存条件: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
阴性对照: CON-HM1NB

数据展示

ELISA Data — Immobilized Biotinylated Human GIPR Nanodisc, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-GIPR Antibody, hFc Tag with the EC50 of 25.5ng/ml determined by ELISA (QC Test). (QC Test)

SPR Data — Biotinylated Human GIPR Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-GIPR Antibody, hFc Tag with an affinity constant of 18.70 nM as determined in SPR assay (Biacore T200).

BLI Data — Loaded Biotinylated Human GIPR Nanodisc, His Tag on Streptavidin-Biosensor can bind Anti-GIPR Antibody, hFc Tag with an affinity constant of 19.60 nM as determined in BLI assay.

背景介绍

The gastric inhibitory polypeptide receptor (GIPR), a G protein-coupled receptor (GPCR) that regulates glucose metabolism and insulin secretion, is a target for the development of therapeutic agents to address type 2 diabetes and obesity.

文献引用

1.Brown KA, Morris RK, Eckhardt SJ, Ge Y, Gellman SH. Phosphorylation Sites of the Gastric Inhibitory Polypeptide Receptor (GIPR) Revealed by Trapped-Ion-Mobility Spectrometry Coupled to Time-of-Flight Mass Spectrometry (TIMS-TOF MS). J Am Chem Soc. 2023 Dec 27;145(51):28030-28037. doi: 10.1021/jacs.3c09078. Epub 2023 Dec 13. PMID: 38091482; PMCID: PMC10842860.

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Biotinylated Human GIPR Nanodisc

产品简介

产品详情

数据展示

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

背景介绍

文献引用

Biotinylated Human GIPR Nanodisc

产品简介

产品详情

数据展示

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

背景介绍

文献引用