Biotinylated Human GIPR Nanodisc

Name: Biotinylated Human GIPR Nanodisc
Brand: Puweituo Bio
SKU: GIPR-HB003

Catalog numberGIPR-HB003

HumanHEK293Molecule · GIP-R; GIPR; PGQTL2Molecular weight · 63.8 kDa

Product introduction

Recombinant Biotinylated Human GIPR Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Cys466 (UniProt accession: P48546-1).

Product details

Molecular aliases: GIP-R; GIPR; PGQTL2
Protein length: Met1-Cys466
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 63.8 kDa.
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Storage: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Negative control: CON-HM1NB

Data display

ELISA Data — Immobilized Biotinylated Human GIPR Nanodisc, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-GIPR Antibody, hFc Tag with the EC50 of 25.5ng/ml determined by ELISA (QC Test). (QC Test)

SPR Data — Biotinylated Human GIPR Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-GIPR Antibody, hFc Tag with an affinity constant of 18.70 nM as determined in SPR assay (Biacore T200).

BLI Data — Loaded Biotinylated Human GIPR Nanodisc, His Tag on Streptavidin-Biosensor can bind Anti-GIPR Antibody, hFc Tag with an affinity constant of 19.60 nM as determined in BLI assay.

Background

The gastric inhibitory polypeptide receptor (GIPR), a G protein-coupled receptor (GPCR) that regulates glucose metabolism and insulin secretion, is a target for the development of therapeutic agents to address type 2 diabetes and obesity.

References

1.Brown KA, Morris RK, Eckhardt SJ, Ge Y, Gellman SH. Phosphorylation Sites of the Gastric Inhibitory Polypeptide Receptor (GIPR) Revealed by Trapped-Ion-Mobility Spectrometry Coupled to Time-of-Flight Mass Spectrometry (TIMS-TOF MS). J Am Chem Soc. 2023 Dec 27;145(51):28030-28037. doi: 10.1021/jacs.3c09078. Epub 2023 Dec 13. PMID: 38091482; PMCID: PMC10842860.

加载中…

Biotinylated Human GIPR Nanodisc

Catalog numberGIPR-HB003

HumanHEK293Molecule · GIP-R; GIPR; PGQTL2Molecular weight · 63.8 kDa

Product introduction

Recombinant Biotinylated Human GIPR Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Cys466 (UniProt accession: P48546-1).

Biotinylated Human GIPR Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

Background

References

Biotinylated Human GIPR Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

Background

References