Biotinylated Human GCGR/Glucagon receptor Nanodisc Protein

Name: Biotinylated Human GCGR/Glucagon receptor Nanodisc Protein
Brand: 普维拓生物
SKU: GCGR-HB001

货号GCGR-HB001

HumanHEK293分子名 · Glucagon receptor

产品简介

Biotinylated Human GCGR/Glucagon receptor Nanodisc Protein is a full-length multi-pass membrane protein presented in a Nanodisc format for antibody discovery, binding assays and method development. Recombinant Biotinylated Human GCGR/Glucagon receptor Nanodisc is expressed from HEK293 with His tag at the C-terminus. It contains Met1-Phe477 (accession: P47871).

产品详情

分子别名: Glucagon receptor; GL-R; GCGR; Glucagon R
蛋白长度: Met1-Phe477
表达系统: HEK293
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
储存条件: Valid for 12 months from date of receipt when stored at -80°C. Aliquot where appropriate and minimize freeze-thaw cycles.

背景介绍

The pancreatic hormone glucagon activates the glucagon receptor (GCGR), a class B seven-transmembrane G protein-coupled receptor that couples to the stimulatory heterotrimeric G protein and provokes PKA-dependent signaling cascades vital to hepatic glucose metabolism and islet insulin secretion. The GCGR expressed at the plasma membrane is constitutively ubiquitinated and upon agonist-activation, internalized GCGRs are deubiquitinated at early endosomes and recycled via Rab4-containing vesicles.

文献引用

1.Kaur S, Sokrat B, Capozzi ME, El K, Bai Y, Jazic A, Han B, Krishna Kumar K, D'Alessio DA, Campbell JE, Bouvier M, Shenoy SK. The ubiquitination status of the glucagon receptor determines signal bias. J Biol Chem. 2023 May;299(5):104690. doi: 10.1016/j.jbc.2023.104690. Epub 2023 Apr 8. PMID: 37037304; PMCID: PMC10200972.

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