Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Tetramer Protein

Name: Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Tetramer Protein
Brand: 普维拓生物
SKU: CMVPP65-HP003

货号CMVPP65-HP003

HumanHEK293分子名 · MHC理论分子量 · 258 kDa

产品简介

Recombinant Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and NLVPMVATV peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &NLVPMVATV).

产品详情

分子别名: MHC; HLA-A*02:01; CMV pp65
蛋白长度: Gly25-Thr305
表达系统: HEK293
理论分子量: The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Tetramer on Bis-Tris PAGE under under Non reduced (N) condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Tetramer is greater than 95% as determined by SEC-HPLC.

背景介绍

Human cytomegalovirus (CMV) is one of the major causes of severe complications in immunocompromised patients, such as recipients of hematopoietic cells, solid organ transplantation, and HIV-infected individuals. CMV is also a major pathogen in congenital infection. It can cause life-threatening diseases and severe neurological sequelae in newborn infants. CMV phosphoprotein pp65 is thought to be a major antigen for CMV-specific cellular immunity.

文献引用

1.Tanaka N, Kimura H, Hoshino Y, Nishikawa K, Kojima S, Nishiyama Y, Morishima T. Expression of tegument protein pp65 of human cytomegalovirus (CMV) and its application to the analysis of viral-specific cellular immunity in CMV-infected individuals. Arch Virol. 2002 Dec;147(12):2405-17. doi: 10.1007/s00705-002-0860-0. PMID: 12491106.

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Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Tetramer Protein

货号CMVPP65-HP003

HumanHEK293分子名 · MHC理论分子量 · 258 kDa

Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Tetramer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

背景介绍

文献引用

Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Tetramer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

背景介绍

文献引用