Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein

Name: Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein
Brand: 普维拓生物
SKU: CMVPP65-HB002

货号CMVPP65-HB002

HumanE. coli分子名 · CMVpp65; NLVPMVATV理论分子量 · 35.6 kDa

产品简介

Recombinant Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and NLVPMVATV peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &NLVPMVATV).

产品详情

分子别名: CMVpp65; NLVPMVATV
蛋白长度: Gly25-Thr305
表达系统: E. coli
理论分子量: The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer, His Tag at 1μg/ml(100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 12.1ng/ml determined by ELISA.

背景介绍

Human cytomegalovirus (CMV), a β-herpes virus with a double-stranded DNA, infects a wide variety of cells and establishes latency in the host. CMVpp65, a tegument protein of the herpes virus CMV, is the main viral antigen found in peripheral blood mononuclear cells (PBMCs) after viral infection and may activate cell-mediated immunity, accounting for 70-90% of the cytotoxic CD8+ T cells' (CTLs) response to CMV. Among the pp65-derived CTL epitope peptides, the 9-mer peptide 495NLVPMVATV503 (CMVpp65 495-503 peptide) is the most immunogenic T cell epitope predominantly displayed on HLA-A*02:01, the most common MHC-I allele in the population.

文献引用

1.[1] Lee SY, Ko DH, Son MJ, Kim JA, Jung K, Kim YS. Affinity Maturation of a T-Cell Receptor-Like Antibody Specific for a Cytomegalovirus pp65-Derived Peptide Presented by HLA-A*02:01. Int J Mol Sci. 2021 Feb 26;22(5):2349. doi: 10.3390/ijms22052349.
2.[2] Hasan AN, Doubrovina E, Sottile R, Prockop S, Klatt MG, Heller G, Selvakumar A, Barnett L, Hsu KC, O'Reilly RJ. Dominant epitopes presented by prevalent HLA alleles permit wide use of banked CMVpp65 T cells in adoptive therapy. Blood Adv. 2022 Aug 23;6(16):4859-4872. doi: 10.1182/bloodadvances.2022007005.
3.[3] Zaia JA, Gallez-Hawkins G, Li X, Yao ZQ, Lomeli N, Molinder K, La Rosa C, Diamond DJ. Infrequent occurrence of natural mutations in the pp65(495-503) epitope sequence presented by the HLA A*0201 allele among human cytomegalovirus isolates. J Virol. 2001 Mar;75(5):2472-4. doi: 10.1128/JVI.75.5.2472-2474.2001.

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Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein

货号CMVPP65-HB002

HumanE. coli分子名 · CMVpp65; NLVPMVATV理论分子量 · 35.6 kDa

Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用

Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用