Biotinylated Human Claudin 6 VLP

Name: Biotinylated Human Claudin 6 VLP
Brand: 普维拓生物
SKU: CLDN6-HB003

货号CLDN6-HB003

HumanHEK293分子名 · Claudin-6理论分子量 · 24.5 kDa

产品简介

Recombinant Biotinylated Full length Human Claudin 6 VLP is expressed in HEK293 cells. It contains amino acid residues Met1-Val220 (It may have cross reaction with anti-His antibody) (UniProt accession: P56747).

产品详情

分子别名: Claudin-6; Skullin; CLDN6; Claudin6; Skullin 2
蛋白长度: Met1-Val220
表达系统: HEK293
理论分子量: The target protein has a predicted MW of 24.5 kDa.
纯度: > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Supplied as 0.22μm filtered solution in PBS, 300mM L-arginine (pH 7.4).
储存条件: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
阴性对照: GPR-HM05CB

数据展示

SEC-HPLC Data — The purity of Biotinylated Human Claudin 6 VLP is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human Claudin 6 VLP at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Claudin6 Antibody, mFc Tag with the EC50 of 6.5ng/ml determined by ELISA (QC Test).

SPR Data — Biotinylated Human Claudin 6 VLP captured on CM5 Chip via streptavidin can bind Anti-Claudin 6 Antibody with an affinity constant of 0.46 nM as determined in SPR assay (Biacore T200).

BLI Data — Loaded Biotinylated Human Claudin 6 VLP on Streptavidin-Biosensor can bind Anti-Claudin 6 Antibody, hFc Tag with an affinity constant of 1.46 nM as determined in BLI assay.

背景介绍

Claudin-6 is a multipass transmembrane protein in the Claudin family. Claudin-6 is expressed by epithelial cells where it participates in tissue development and the maintenance of tight junction integrity. Human Claudin-6 shares 88% and 86% amino acid sequence identity with mouse and rat Claudin-6, respectively.

文献引用

1.Sugimoto K, Ichikawa-Tomikawa N, Kashiwagi K, Endo C, Tanaka S, Sawada N, Watabe T, Higashi T, Chiba H. Cell adhesion signals regulate the nuclear receptor activity. Proc Natl Acad Sci U S A. 2019 Dec 3;116(49):24600-24609. doi: 10.1073/pnas.1913346116. Epub 2019 Nov 18. PMID: 31740618; PMCID: PMC6900646.

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Biotinylated Human Claudin 6 VLP

货号CLDN6-HB003

HumanHEK293分子名 · Claudin-6理论分子量 · 24.5 kDa

Biotinylated Human Claudin 6 VLP

产品简介

产品详情

数据展示

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

背景介绍

文献引用

Biotinylated Human Claudin 6 VLP

产品简介

产品详情

数据展示

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

背景介绍

文献引用