Biotinylated Human Claudin 6 VLP

Name: Biotinylated Human Claudin 6 VLP
Brand: Puweituo Bio
SKU: CLDN6-HB003

Catalog numberCLDN6-HB003

HumanHEK293Molecule · Claudin-6Molecular weight · 24.5 kDa

Product introduction

Recombinant Biotinylated Full length Human Claudin 6 VLP is expressed in HEK293 cells. It contains amino acid residues Met1-Val220 (It may have cross reaction with anti-His antibody) (UniProt accession: P56747).

Product details

Molecular aliases: Claudin-6; Skullin; CLDN6; Claudin6; Skullin 2
Protein length: Met1-Val220
Expression system: HEK293
Molecular weight: The target protein has a predicted MW of 24.5 kDa.
Purity: > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22μm filtered solution in PBS, 300mM L-arginine (pH 7.4).
Storage: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Negative control: GPR-HM05CB

Data display

SEC-HPLC Data — The purity of Biotinylated Human Claudin 6 VLP is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human Claudin 6 VLP at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Claudin6 Antibody, mFc Tag with the EC50 of 6.5ng/ml determined by ELISA (QC Test).

SPR Data — Biotinylated Human Claudin 6 VLP captured on CM5 Chip via streptavidin can bind Anti-Claudin 6 Antibody with an affinity constant of 0.46 nM as determined in SPR assay (Biacore T200).

BLI Data — Loaded Biotinylated Human Claudin 6 VLP on Streptavidin-Biosensor can bind Anti-Claudin 6 Antibody, hFc Tag with an affinity constant of 1.46 nM as determined in BLI assay.

Background

Claudin-6 is a multipass transmembrane protein in the Claudin family. Claudin-6 is expressed by epithelial cells where it participates in tissue development and the maintenance of tight junction integrity. Human Claudin-6 shares 88% and 86% amino acid sequence identity with mouse and rat Claudin-6, respectively.

References

1.Sugimoto K, Ichikawa-Tomikawa N, Kashiwagi K, Endo C, Tanaka S, Sawada N, Watabe T, Higashi T, Chiba H. Cell adhesion signals regulate the nuclear receptor activity. Proc Natl Acad Sci U S A. 2019 Dec 3;116(49):24600-24609. doi: 10.1073/pnas.1913346116. Epub 2019 Nov 18. PMID: 31740618; PMCID: PMC6900646.

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Biotinylated Human Claudin 6 VLP

Catalog numberCLDN6-HB003

HumanHEK293Molecule · Claudin-6Molecular weight · 24.5 kDa

Biotinylated Human Claudin 6 VLP

Product introduction

Product details

Data display

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

Background

References

Biotinylated Human Claudin 6 VLP

Product introduction

Product details

Data display

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

Background

References