Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein (Primary Amine Labeling)

Name: Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein (Primary Amine Labeling)
Brand: 普维拓生物
SKU: CD40-HB004

货号CD40-HB004

HumanHEK293分子名 · CD40L理论分子量 · 52.4 kDa

产品简介

Recombinant Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein (Primary Amine Labeling) is expressed in HEK293 cells with a His tag and Flag tag at the N-terminus. It contains amino acid residues Met113-Leu261 trimer design (accession: P29965(Human) / G7Q1T4(Cynomolgus) / P63304(Rhesus macaque)).

产品详情

分子别名: CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand
蛋白长度: Met113-Leu261
表达系统: HEK293
理论分子量: The protein has a predicted MW of 52.4 kDa, Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human CD40, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer, His Tag with the EC50 of 47.6ng/ml determined by ELISA (QC Test).

背景介绍

CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.

文献引用

1.Bjorck P, Filbert E, Zhang Y, et al. The CD40 agonistic monoclonal antibody APX005M has potent immune stimulatory capabilities[J]. Journal for Immunotherapy of Cancer, 2015, 3(S2): P198.

加载中…

Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein (Primary Amine Labeling)

货号CD40-HB004

HumanHEK293分子名 · CD40L理论分子量 · 52.4 kDa

Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein (Primary Amine Labeling)

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用

Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein (Primary Amine Labeling)

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用