Mouse CD27 Ligand/CD70 Protein

Name: Mouse CD27 Ligand/CD70 Protein
Brand: 普维拓生物
SKU: CD27-MF002

货号CD27-MF002

MouseHEK293分子名 · CD70 molecule理论分子量 · 43.41 kDa

产品简介

Recombinant Mouse CD27 Ligand/CD70 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the N-terminus. It contains amino acid residues Ser45-Pro195 (UniProt accession: O55237).

产品详情

分子别名: CD70 molecule; CD70; TNFSF7; CD27 Ligand; CD27-L; CD27LG;-Ki-24 antigen; TNFSF7G;CD27L; -Ki24 antigen
蛋白长度: Ser45-Pro195
表达系统: HEK293
理论分子量: The protein has a predicted MW of 43.41 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素: Less than 1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22μm filtered solution in PBS (pH 6.2). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Mouse CD27 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Mouse CD27 Ligand is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Mouse CD27, mFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 34.0ng/ml determined by ELISA.

背景介绍

CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7. CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.

文献引用

1.Buchan S L, Rogel A, Al-Shamkhani A. The immunobiology of CD27 and OX40 and their potential as targets for cancer immunotherapy[J]. Blood, 2017:blood-2017-07-741025. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.

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Mouse CD27 Ligand/CD70 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用

Mouse CD27 Ligand/CD70 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

背景介绍

文献引用