Biotinylated Human CD133 Nanodisc

Name: Biotinylated Human CD133 Nanodisc
Brand: 普维拓生物
SKU: CD133-HB001

货号CD133-HB001

HumanHEK293分子名 · PROM1理论分子量 · 110.1 kDa

产品简介

Recombinant Biotinylated Human CD133 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-His865 (UniProt accession: O43490-1).

产品详情

分子别名: PROM1; PROML1; MSTP061; Prominin-1; AC133
蛋白长度: Met1-His865
表达系统: HEK293
理论分子量: The protein has a predicted MW of 110.1 kDa.
内毒素: Less than 1EU per μg by the LAL method.
缓冲液 / 制剂: Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
储存条件: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
阴性对照: CON-HM1NB

数据展示

ELISA Data — Immobilized Biotinylated Human CD133 Nanodisc, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD133 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA. (QC Test)

SPR Data — Biotinylated Biotinylated Human CD133 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-CD133 Antibody, hFc Tag with an affinity constant of 26.57 pM as determined in SPR assay (Biacore T200).

背景介绍

Prominin-1 (PROM1), also known as CD133, is expressed in hepatic progenitor cells (HPCs) and cholangiocytes of the fibrotic liver. It is a cell surface biomarker that allows the identification of stem and cancer stem cells from different organs. It is also expressed in several differentiated epithelial and non-epithelial cells.

文献引用

1.Thamm K, Šimaitė D, Karbanová J, Bermúdez V, Reichert D, Morgenstern A, Bornhäuser M, Huttner WB, Wilsch-Bräuninger M, Corbeil D. Prominin-1 (CD133) modulates the architecture and dynamics of microvilli. Traffic. 2019 Jan;20(1):39-60. doi: 10.1111/tra.12618. Epub 2018 Nov 14. PMID: 30328220.
2.Lee H, Yu DM, Bahn MS, Kwon YJ, Um MJ, Yoon SY, Kim KT, Lee MW, Jo SJ, Lee S, Koo SH, Jung KH, Lee JS, Ko YG. Hepatocyte-specific Prominin-1 protects against liver injury-induced fibrosis by stabilizing SMAD7. Exp Mol Med. 2022 Aug;54(8):1277-1289. doi: 10.1038/s12276-022-00831-y. Epub 2022 Aug 29. PMID: 36038590; PMCID: PMC9440255.

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Biotinylated Human CD133 Nanodisc

产品简介

产品详情

数据展示

ELISA

ELISA Data

SPR

SPR Data

背景介绍

文献引用

Biotinylated Human CD133 Nanodisc

产品简介

产品详情

数据展示

ELISA

ELISA Data

SPR

SPR Data

背景介绍

文献引用