Biotinylated Human CD133 Nanodisc

Name: Biotinylated Human CD133 Nanodisc
Brand: Puweituo Bio
SKU: CD133-HB001

Catalog numberCD133-HB001

HumanHEK293Molecule · PROM1Molecular weight · 110.1 kDa

Product introduction

Recombinant Biotinylated Human CD133 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-His865 (UniProt accession: O43490-1).

Product details

Molecular aliases: PROM1; PROML1; MSTP061; Prominin-1; AC133
Protein length: Met1-His865
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 110.1 kDa.
Endotoxin: Less than 1EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Storage: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Negative control: CON-HM1NB

Data display

ELISA Data — Immobilized Biotinylated Human CD133 Nanodisc, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD133 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA. (QC Test)

SPR Data — Biotinylated Biotinylated Human CD133 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-CD133 Antibody, hFc Tag with an affinity constant of 26.57 pM as determined in SPR assay (Biacore T200).

Background

Prominin-1 (PROM1), also known as CD133, is expressed in hepatic progenitor cells (HPCs) and cholangiocytes of the fibrotic liver. It is a cell surface biomarker that allows the identification of stem and cancer stem cells from different organs. It is also expressed in several differentiated epithelial and non-epithelial cells.

References

1.Thamm K, Šimaitė D, Karbanová J, Bermúdez V, Reichert D, Morgenstern A, Bornhäuser M, Huttner WB, Wilsch-Bräuninger M, Corbeil D. Prominin-1 (CD133) modulates the architecture and dynamics of microvilli. Traffic. 2019 Jan;20(1):39-60. doi: 10.1111/tra.12618. Epub 2018 Nov 14. PMID: 30328220.
2.Lee H, Yu DM, Bahn MS, Kwon YJ, Um MJ, Yoon SY, Kim KT, Lee MW, Jo SJ, Lee S, Koo SH, Jung KH, Lee JS, Ko YG. Hepatocyte-specific Prominin-1 protects against liver injury-induced fibrosis by stabilizing SMAD7. Exp Mol Med. 2022 Aug;54(8):1277-1289. doi: 10.1038/s12276-022-00831-y. Epub 2022 Aug 29. PMID: 36038590; PMCID: PMC9440255.

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Biotinylated Human CD133 Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

SPR

SPR Data

Background

References

Biotinylated Human CD133 Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

SPR

SPR Data

Background

References