Human IL-5 R alpha/CD125 Protein

Name: Human IL-5 R alpha/CD125 Protein
Brand: 普维拓生物
SKU: CD125-HF001

货号CD125-HF001

HumanHEK293分子名 · CD125理论分子量 · 62.73 kDa

产品简介

Recombinant Human IL-5 R alpha/CD125 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the N-terminus. It contains amino acid residues Asp21-Trp342 (UniProt accession: Q01344-1).

产品详情

分子别名: CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; MGC26560; IL5R alpha; IL5R subunit alpha
蛋白长度: Asp21-Trp342
表达系统: HEK293
理论分子量: The protein has a predicted MW of 62.73 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on Bis-Tris PAGE result.
纯度: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
内毒素: Less than 0.1 EU per μg by the LAL method.
缓冲液 / 制剂: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
状态: 冻干粉
储存条件: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
复溶建议: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

数据展示

Bis-Tris PAGE Data — Human IL-5 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human IL-5 R alpha is greater than 90% as determined by SEC-HPLC.

ELISA Data — Immobilized Human IL-5, His Tag (Cat. IL5-HM101) at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. (QC Test)

BLI Data — Loaded Human IL-5 R alpha, hFc Tag on ProA-Biosensor can bind Human IL-5, His Tag with an affinity constant of 14.80 nM as determined in BLI assay.

背景介绍

Interleukin‑5 Receptor alpha (IL‑5 R alpha), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology. This is the receptor for interleukin-5. The alpha chain binds to IL5.

文献引用

1.Garcia G, Taille C, Laveneziana P, et al. Anti-interleukin-5 therapy in severe asthma[J]. European Respiratory Review, 2013, 22(129):251-257.

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Human IL-5 R alpha/CD125 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

BLI

BLI Data

背景介绍

文献引用

Human IL-5 R alpha/CD125 Protein

产品简介

产品详情

数据展示

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

BLI

BLI Data

背景介绍

文献引用